Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations [0]
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- Product number
- 50-4778-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-FOXA2 Monoclonal Antibody (3C10), eFluor 660, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This 3C10 monoclonal antibody reacts with human FOXA2, which is also known as hepatocyte nuclear factor 3 beta (HNF3B). This 48-kDa member of the Forkhead family of transcription factors is expressed in the liver, pancreas (islets and some acinar cells), lung, and intestine. During development, FOXA2 is necessary for lung bud and pancreatic beta cell formation. Recently, directed expression of FOXA2 in embryonic stem cells has been found to regulate the generation and differentiation of midbrain dopaminergic neurons. It has also been shown to regulate genes involved hepatic lipid metabolism, including alpha fetoprotein, albumin, and transthyretin, as well as Pdx1 in the pancreas. FOXA2 expression is negatively regulated by insulin and reports demonstrate a role for it in sporadic cases of maturity-onset diabetes of the young and the epithelial-to-mesenchymal transition in cancer. Applications Reported: This 3C10 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. Applications Tested: This 3C10 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3C10
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Loss of FOXA1/2 is essential for the epithelial-to-mesenchymal transition in pancreatic cancer.
Nuclear export-independent inhibition of Foxa2 by insulin.
Foxa2 regulates lipid metabolism and ketogenesis in the liver during fasting and in diabetes.
The hepatocyte nuclear factor 3 (HNF3 or FOXA) family in metabolism.
Song Y, Washington MK, Crawford HC
Cancer research 2010 Mar 1;70(5):2115-25
Cancer research 2010 Mar 1;70(5):2115-25
Nuclear export-independent inhibition of Foxa2 by insulin.
Howell JJ, Stoffel M
The Journal of biological chemistry 2009 Sep 11;284(37):24816-24
The Journal of biological chemistry 2009 Sep 11;284(37):24816-24
Foxa2 regulates lipid metabolism and ketogenesis in the liver during fasting and in diabetes.
Wolfrum C, Asilmaz E, Luca E, Friedman JM, Stoffel M
Nature 2004 Dec 23;432(7020):1027-32
Nature 2004 Dec 23;432(7020):1027-32
The hepatocyte nuclear factor 3 (HNF3 or FOXA) family in metabolism.
Kaestner KH
Trends in endocrinology and metabolism: TEM 2000 Sep;11(7):281-5
Trends in endocrinology and metabolism: TEM 2000 Sep;11(7):281-5
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