Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- ABIN952361 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Forkhead Box A2 (FOXA2) (AA 135-164), (Middle Region) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 135-164 amino acids from the Central region of human FOXA2 / HNF3B
- Description
- Protein A column, followed by peptide affinity purification
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- AA 135-164,Middle Region
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.
Foxa2 (HNF-3beta) regulates expression of hepatotrophic factor ALR in liver cells.
Hepatitis C virus differentially modulates activation of forkhead transcription factors and insulin-induced metabolic gene expression.
Bailey SD, Xie C, Do R, Montpetit A, Diaz R, Mohan V, Keavney B, Yusuf S, Gerstein HC, Engert JC, Anand S, DREAM investigators
Diabetes care 2010 Oct;33(10):2250-3
Diabetes care 2010 Oct;33(10):2250-3
Foxa2 (HNF-3beta) regulates expression of hepatotrophic factor ALR in liver cells.
Dayoub R, Groitl P, Dobner T, Bosserhoff AK, Schlitt HJ, Weiss TS
Biochemical and biophysical research communications 2010 May 14;395(4):465-70
Biochemical and biophysical research communications 2010 May 14;395(4):465-70
Hepatitis C virus differentially modulates activation of forkhead transcription factors and insulin-induced metabolic gene expression.
Banerjee A, Meyer K, Mazumdar B, Ray RB, Ray R
Journal of virology 2010 Jun;84(12):5936-46
Journal of virology 2010 Jun;84(12):5936-46
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of FOXA2 / HNF3B Antibody (Center) Cat.-No AP51702PU-N in MDA-MB231 cell line lysates (35ug/lane). This demonstrates the FOXA2 antibody detected the FOXA2 protein (arrow).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of FOXA2 / HNF3B Antibody (Center) Cat.-No AP51702PU-N with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue reacted with FOXA2 / HNF3B Antibody (Center) Cat.-No AP51702PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.