14-5923-80

antibody from Invitrogen Antibodies

Targeting: SOX10 DOM, WS2E, WS4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

14-5923-80

Provider

Invitrogen Antibodies

Product name

SOX10 Monoclonal Antibody (20B7), eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: The monoclonal antibody 20B7 recognizes Sox10, is a member of the Sox superfamily (SRY-related HMG-box) that contain a DNA binding domain (high-mobility group domain) and functions as a transcription factor. Initially, Sox10 expression is localized to the developing neural crest and peripheral nervous system, but later is expressed in glial progenitors and mature oligodendrocytes. Sox10 is involved in stem cell maintenance, melanocyte differentiation, as well as central and peripheral gliogenesis and neurogenesis. Sox10 regulation of downstream proteins such as MASH1, PHOX2B, PDGF-alpha, and MITF is dependent on expression level. Sox10 is also expressed in multipotent vascular stem cells, melanomas, and gliomas.

Antibody clone number

20B7

Concentration

0.5 mg/mL

References

Submitted references

Early embryonic development and spatiotemporal localization of mammalian primordial germ cell-associated proteins in the basal rodent Lagostomus maximus.

Leopardo NP, Vitullo AD
Scientific reports 2017 Apr 4;7(1):594

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Sox10 has a broad expression pattern in gliomas and enhances platelet-derived growth factor-B--induced gliomagenesis.

Ferletta M, Uhrbom L, Olofsson T, Pontén F, Westermark B
Molecular cancer research : MCR 2007 Sep;5(9):891-7

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SOX10 maintains multipotency and inhibits neuronal differentiation of neural crest stem cells.

Kim J, Lo L, Dormand E, Anderson DJ
Neuron 2003 Apr 10;38(1):17-31

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Comparison of the generic neuronal differentiation and neuron subtype specification functions of mammalian achaete-scute and atonal homologs in cultured neural progenitor cells.

Lo L, Dormand E, Greenwood A, Anderson DJ
Development (Cambridge, England) 2002 Apr;129(7):1553-67

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-SOX10 Monoclonal Antibody (20B7), eBioscience (Product # 14-5923-82) and a 58 kDa band corresponding to SOX10 was observed across the cell line tested. Nuclear enriched extracts (30 µg lysate) of A-375 (Lane 1) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1ug/ml) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of SOX10 was performed using 70% confluent log phase A-375 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with SOX10 Monoclonal Antibody (20B7), eBioscience (Product # 14-5923-82) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of formalin-fixed paraffin embedded human cerebellum stained with 10 µg/mL of Mouse IgG1 K Isotype Control Purified (Product # 14-4714-82) (left) or 10 µg/mL of Anti-Sox10 Purified (right) followed by Anti-Mouse IgG Biotin, Streptavidin HRP, and DAB visualization.Nuclei are counterstained with hematoxylin.