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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PAB4550 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4550, RRID:AB_1571068
- Product name
- AKT2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of AKT2.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references A family with severe insulin resistance and diabetes due to a mutation in AKT2.
Protein kinase B inhibits apoptosis induced by actinomycin D in ECV304 cells through phosphorylation of caspase 8.
Akt2 expression correlates with prognosis of human hepatocellular carcinoma.
George S, Rochford JJ, Wolfrum C, Gray SL, Schinner S, Wilson JC, Soos MA, Murgatroyd PR, Williams RM, Acerini CL, Dunger DB, Barford D, Umpleby AM, Wareham NJ, Davies HA, Schafer AJ, Stoffel M, O'Rahilly S, Barroso I
Science (New York, N.Y.) 2004 May 28;304(5675):1325-8
Science (New York, N.Y.) 2004 May 28;304(5675):1325-8
Protein kinase B inhibits apoptosis induced by actinomycin D in ECV304 cells through phosphorylation of caspase 8.
Shim D, Kang HY, Jeon BW, Kang SS, Chang SI, Kim HY
Archives of biochemistry and biophysics 2004 May 15;425(2):214-20
Archives of biochemistry and biophysics 2004 May 15;425(2):214-20
Akt2 expression correlates with prognosis of human hepatocellular carcinoma.
Xu X, Sakon M, Nagano H, Hiraoka N, Yamamoto H, Hayashi N, Dono K, Nakamori S, Umeshita K, Ito Y, Matsuura N, Monden M
Oncology reports 2004 Jan;11(1):25-32
Oncology reports 2004 Jan;11(1):25-32
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of AKT2 polyclonal antibody (Cat # PAB4550) in 293 cell line lysates transiently transfected with the AKT2 gene (2 ug/lane). AKT2 (arrow) was detected using the purified AKT2 polyclonal antibody (Cat # PAB4550) (1 : 60 dilution).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of AKT2 polyclonal antibody (Cat # PAB4550) in mouse liver tissue lysates (35 ug/lane). AKT2 (arrow) was detected using the purified AKT2 polyclonal antibody (Cat # PAB4550) (1 : 60 dilution).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human colon carcinoma reacted with AKT2 polyclonal antibody (Cat # PAB4550) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated .
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
