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- References [4]
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- Product number
- ABIN359081 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (Ser474) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S474 of human AKT2.
- Description
- Affinity chromatography on Protein A
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- Ser474
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references A family with severe insulin resistance and diabetes due to a mutation in AKT2.
Protein kinase B inhibits apoptosis induced by actinomycin D in ECV304 cells through phosphorylation of caspase 8.
Akt2, phosphatidylinositol 3-kinase, and PTEN are in lipid rafts of intestinal cells: role in absorption and differentiation.
Akt2 expression correlates with prognosis of human hepatocellular carcinoma.
George S, Rochford JJ, Wolfrum C, Gray SL, Schinner S, Wilson JC, Soos MA, Murgatroyd PR, Williams RM, Acerini CL, Dunger DB, Barford D, Umpleby AM, Wareham NJ, Davies HA, Schafer AJ, Stoffel M, O'Rahilly S, Barroso I
Science (New York, N.Y.) 2004 May 28;304(5675):1325-8
Science (New York, N.Y.) 2004 May 28;304(5675):1325-8
Protein kinase B inhibits apoptosis induced by actinomycin D in ECV304 cells through phosphorylation of caspase 8.
Shim D, Kang HY, Jeon BW, Kang SS, Chang SI, Kim HY
Archives of biochemistry and biophysics 2004 May 15;425(2):214-20
Archives of biochemistry and biophysics 2004 May 15;425(2):214-20
Akt2, phosphatidylinositol 3-kinase, and PTEN are in lipid rafts of intestinal cells: role in absorption and differentiation.
Li X, Leu S, Cheong A, Zhang H, Baibakov B, Shih C, Birnbaum MJ, Donowitz M
Gastroenterology 2004 Jan;126(1):122-35
Gastroenterology 2004 Jan;126(1):122-35
Akt2 expression correlates with prognosis of human hepatocellular carcinoma.
Xu X, Sakon M, Nagano H, Hiraoka N, Yamamoto H, Hayashi N, Dono K, Nakamori S, Umeshita K, Ito Y, Matsuura N, Monden M
Oncology reports 2004 Jan;11(1):25-32
Oncology reports 2004 Jan;11(1):25-32
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of AKT2 (arrow) using rabbit polyclonal AKT2 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AKT2 gene (Lane 2)
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AKT2 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
