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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Product number
- ABIN358087 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-BCL2-Associated Agonist of Cell Death (BAD) (pSer99) antibody
- Antibody type
- Polyclonal
- Description
- Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- pSer99
- Vial size
- 0.4 mL
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Cooperation of amphiregulin and insulin-like growth factor-1 inhibits Bax- and Bad-mediated apoptosis via a protein kinase C-dependent pathway in non-small cell lung cancer cells.
Broad degradation of proapoptotic proteins with the conserved Bcl-2 homology domain 3 during infection with Chlamydia trachomatis.
A chimeric protein induces tumor cell apoptosis by delivering the human Bcl-2 family BH3-only protein Bad.
BAD is a pro-survival factor prior to activation of its pro-apoptotic function.
Inactivating mutations of proapoptotic Bad gene in human colon cancers.
Hurbin A, Coll JL, Dubrez-Daloz L, Mari B, Auberger P, Brambilla C, Favrot MC
The Journal of biological chemistry 2005 May 20;280(20):19757-67
The Journal of biological chemistry 2005 May 20;280(20):19757-67
Broad degradation of proapoptotic proteins with the conserved Bcl-2 homology domain 3 during infection with Chlamydia trachomatis.
Ying S, Seiffert BM, Häcker G, Fischer SF
Infection and immunity 2005 Mar;73(3):1399-403
Infection and immunity 2005 Mar;73(3):1399-403
A chimeric protein induces tumor cell apoptosis by delivering the human Bcl-2 family BH3-only protein Bad.
Antignani A, Youle RJ
Biochemistry 2005 Mar 15;44(10):4074-82
Biochemistry 2005 Mar 15;44(10):4074-82
BAD is a pro-survival factor prior to activation of its pro-apoptotic function.
Seo SY, Chen YB, Ivanovska I, Ranger AM, Hong SJ, Dawson VL, Korsmeyer SJ, Bellows DS, Fannjiang Y, Hardwick JM
The Journal of biological chemistry 2004 Oct 1;279(40):42240-9
The Journal of biological chemistry 2004 Oct 1;279(40):42240-9
Inactivating mutations of proapoptotic Bad gene in human colon cancers.
Lee JW, Soung YH, Kim SY, Nam SW, Kim CJ, Cho YG, Lee JH, Kim HS, Park WS, Kim SH, Lee JY, Yoo NJ, Lee SH
Carcinogenesis 2004 Aug;25(8):1371-6
Carcinogenesis 2004 Aug;25(8):1371-6
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