TA324071

antibody from OriGene

Targeting: DOK1 p62dok

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA324071

Provider

OriGene

Product name

Anti-DOK1 (phospho-Tyr362) Rabbit Polyclonal Antibody

Antibody type

Polyclonal

Description

Anti-DOK1 (phospho-Tyr362) Rabbit Polyclonal Antibody

Host

Rabbit

Conjugate

Unconjugated

Epitope

DOK1

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µl

Concentration

1mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted band size: 62 kDa . Positive control: Jurkat cells treated with insulin lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: Jurkat cells untreated with insulin lysate Lane2: Jurkat cells treated with insulin lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted cell location: Cytoplasm. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using DOK1 (phospho-Tyr362) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification: x200)

Validation comment

IHC