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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- PA5-37828 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-ERK1/ERK2 (Thr202, Tyr204) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control for Western blot is NIH-3T3 cells.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Puerarin attenuates hypoxia-resulted damages in neural stem cells by up-regulating microRNA-214.
Phylogenetically Defined Isoforms of Listeria monocytogenes Invasion Factor InlB Differently Activate Intracellular Signaling Pathways and Interact with the Receptor gC1q-R.
Melatonin reduces intramuscular fat deposition by promoting lipolysis and increasing mitochondrial function.
Wang B, Ma W, Yang H
Artificial cells, nanomedicine, and biotechnology 2019 Dec;47(1):2746-2753
Artificial cells, nanomedicine, and biotechnology 2019 Dec;47(1):2746-2753
Phylogenetically Defined Isoforms of Listeria monocytogenes Invasion Factor InlB Differently Activate Intracellular Signaling Pathways and Interact with the Receptor gC1q-R.
Chalenko Y, Kalinin E, Marchenkov V, Sysolyatina E, Surin A, Sobyanin K, Ermolaeva S
International journal of molecular sciences 2019 Aug 24;20(17)
International journal of molecular sciences 2019 Aug 24;20(17)
Melatonin reduces intramuscular fat deposition by promoting lipolysis and increasing mitochondrial function.
Liu K, Yu W, Wei W, Zhang X, Tian Y, Sherif M, Liu X, Dong C, Wu W, Zhang L, Chen J
Journal of lipid research 2019 Apr;60(4):767-782
Journal of lipid research 2019 Apr;60(4):767-782
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from NIH-3T3 cells treated with IFNa using p44/42 MAP Kinase (pThr202/Tyr204) polyclonal antibody (Product # PA5-37828) compared to the same antibody preincubated with a blocking peptide (Lane 1).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of Phospho-ERK1/ERK2 (Thr202, Tyr204) in mouse-spleen tissue. The tissue was stained using Phospho-ERK1/ERK2 (Thr202, Tyr204) Polyclonal Antibody (Product # PA5-37828)(red) at a 1:200 dilution (4°C,overnight). A Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50 min).Picture B: DAPI (blue) 10 min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-ERK1/ERK2 (Thr202, Tyr204) in paraffin-embedded Rat-lung tissue. 1) Phospho-ERK1/ERK2 (Thr202, Tyr204) Polyclonal Antibody (Product # PA5-37828) was diluted at 1:200 (4°C,overnight). 2) Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20 min). 3)Secondary antibody was diluted at 1:200(room tempeRature, 30 min). Negative control was used by secondary antibody only.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Kinetics of Erk1/2 and Akt phosphorylation in the presence of idInlBs. A total of 100 ng/mL idInlBs were added to HEp-2 cells. Cells were lysed at pointed time points and probed with anti-phospho-Erk1/2 and anti-phospho-Akt antibodies. The curves show digitized data from three independent experiments. * p < 0.05.
