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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunoprecipitation [1]
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- Product number
- NB100-61065 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-61065, RRID:AB_925441
- Product name
- Rabbit Polyclonal HDAC6 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HDAC6 Antibody [NB100-61065] - Detection of Human HDAC6 on HeLa whole cell lysate using NB100-61065. HDAC6 was also immunoprecipitated by rabbit anti-HDAC6 antibody NB100-61064.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HDAC6 Antibody [NB100-61065] - Detection of Human HDAC6 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HDAC6 antibody NB100-61065 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HDAC6 Antibody [NB100-61065] - Whole cell lysate (50 ug) from TCMK-1 and NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HDAC6 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: HDAC6 Antibody [NB100-61065] - Detection of human HDAC6 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HDAC6 antibody NB100-61065 (lot NB100-61065-2) used for IP at 6 ug per reaction. HDAC6 was also immunoprecipitated by a previous lot of this antibody NB100-61065-1. For blotting immunoprecipitated HDAC6,NB100-61065 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
