PA5-22308
antibody from Invitrogen Antibodies
Targeting: LAMC2
BM600-100kDa, EBR2, EBR2A, kalinin-105kDa, LAMB2T, LAMNB2, nicein-100kDa
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-22308 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Laminin gamma-2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Raji, NCI-H929.
- Concentration
- 0.66 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Laminin gamma-2 using A) 30 µg Raji whole cell lysate and B) 30 µg NCI-H929 whole cell lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a Laminin gamma-2 polyclonal antibody (Product # PA5-22308) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Laminin gamma-2 was performed by separating 30 µg of whole cell extract by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Laminin gamma-2 Polyclonal Antibody (Product # PA5-22308) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using Laminin gamma 2 (Product # PA5-22308) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.