TA322862

antibody from OriGene

Targeting: SUMO1 GMP1, OFC10, PIC1, SMT3C, SMT3H3, SUMO-1, UBL1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA322862

Provider

OriGene

Product name

Anti-SUMO1 Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding to a region derived from 56-70 amino acids of Human SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)

Description

Anti-SUMO1 Rabbit Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µl

Concentration

0.7mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted band size: 12 kDa . Positive control: Jukart and A549 cell lysate. Recommended dilution: 1/100-500. (Gel: %SDS-PAGE Lane1: Jukart cell lysate Lane2: A549 cell lysate Lysates: 40 ug per lane Primary antibody: 1/350 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 minutes)

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted cell location: Cytoplasm, Nucleus. Positive control: Human lung cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using SUMO1 antibody at dilution 1/20, on the right is treated with the synthetic peptide. (Original magnification:x200)

Validation comment

IHC