MA3-088

antibody from Invitrogen Antibodies

Targeting: SUMO1 GMP1, OFC10, PIC1, SMT3C, SMT3H3, SUMO-1, UBL1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA3-088

Provider

Invitrogen Antibodies

Product name

SUMO1 Monoclonal Antibody (1SUM-1C6)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

MA3-088 detects SUMO1 from human and mouse samples. The immunogen sequence is conserved in SUMO1 of mouse origin.

Antibody clone number

1SUM-1C6

Concentration

Conc. Not Determined

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SUMO1 was performed by loading 20 µg of the indicated whole cell lysate and 5 µL of PageRuler Plus Prestained Protein Ladder (Product # 26619) per well onto a 4-20% Tris-Glycine polyacrylamide gel (Product # WT4202BX10). Proteins were transferred to a nitrocellulose membrane using the G2 Blotter (Product # 62288), and blocked with 5% Milk in TBST for 1 hour at room temperature. SUMO1-RanGAP was detected at ~80 kDa using a SUMO1 mouse monoclonal antibody (Product # MA3-088) at a dilution of 1:500 in blocking buffer for 1 hour at room temperature on a rocking platform, followed by a Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177) at a dilution of 1:1000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico substrate (Product # 34078) and the myECL Imager (Product # 62236).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SUMO1 (green) in U2OS cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a SUMO1 mouse monoclonal antibody (Product # MA3-088) at a dilution of 1:100 in staining buffer for 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG Superclonal™ Secondary Antibody, Alexa Fluor 488 conjugate (Product # A28175) at a dilution of 1:1000 for 1 hour at room temperature. Nuclei (blue) were counterstained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.