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LearnPAB10319
antibody from Abnova Corporation
Targeting: SUMO1
GMP1, OFC10, PIC1, SMT3C, SMT3H3, SUMO-1, UBL1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- PAB10319 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10319, RRID:AB_1715102
- Product name
- SUMO1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against a recombinant SUMO1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Involvement of PIAS1 in the sumoylation of tumor suppressor p53.
SP-RING for SUMO: new functions bloom for a ubiquitin-like protein.
SUMO, ubiquitin's mysterious cousin.
Kahyo T, Nishida T, Yasuda H
Molecular cell 2001 Sep;8(3):713-8
Molecular cell 2001 Sep;8(3):713-8
SP-RING for SUMO: new functions bloom for a ubiquitin-like protein.
Hochstrasser M
Cell 2001 Oct 5;107(1):5-8
Cell 2001 Oct 5;107(1):5-8
SUMO, ubiquitin's mysterious cousin.
Müller S, Hoege C, Pyrowolakis G, Jentsch S
Nature reviews. Molecular cell biology 2001 Mar;2(3):202-10
Nature reviews. Molecular cell biology 2001 Mar;2(3):202-10
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Supportive validation
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- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoblot of SUMO1 polyclonal antibody (Cat # PAB10319), generated by immunizationwith recombinant human SUMO, was tested by immunoblot against a SUMO-GFP fusion proteinafter cleavage by proteases.Dilution of the antibody between 1 : 1,000 and 1 : 5,000 showed strong reactivity specifically with the SUMO portion of the fusion protein (arrowhead).In this blot the antibody was used at a 1 : 2000 dilution incubated overnight at 4°C in 5% non-fat dry milk in TTBS.Detection occurred using a 1 : 2000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature.Achemiluminescence system was used for signal detection.Data contributed by M.Malakhov, www.lifesensors.com, personal communication.
