R1198

antibody from Acris Antibodies GmbH

Targeting: SUMO1 GMP1, OFC10, PIC1, SMT3C, SMT3H3, SUMO-1, UBL1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

R1198

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#R1198, RRID:AB_1007583

Product name

anti SUMO1

Antibody type

Polyclonal

Antigen

Recombinant yeast SUMO protein.

Reactivity

Yeast

Host

Rabbit

Vial size

0.5 mg

Concentration

1.0 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure. Immunoblot of SUMO-GFP fusion proteins cleaved by insect cell protein extracts. R1198 SUMO antibody is generated by immunization with recombinant Yeast SUMO, was tested by Immunoblot against several constructs of SUMO-GFP fusion proteins after cleavage by proteases in insect cell protein extracts. These constructs contained various linkers between the SUMO and GFP portion of the fusion proteins. Each sample was run twice. The left Lanes each contain 2 µg E.coli expressed and purified SUMO-GFP fusion proteins after incubation with lysed cells (50 µg total protein) for 1 h. The right Lanes contain the same fusion proteins incubated with the lysate in the presence of 2% SDS. After probing with anti-GFP antibodies the membranes were stripped of antibody using SDS-DTT solution for 30 mn at 60°C and were then re-probed using the R1198 SUMO antibody at a 1/1000 dilution incubated overnight at 4°C in 5% non-fat dry milk in TTBS. Detection occurred using a 1/2000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 h at RT. A chemiluminescence system was used for signal detection (Roche). Other detection systems will yield similar results.