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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- TA319220 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal PPAR Gamma 2 antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal PPAR Gamma 2 antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- PPARG
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 1.0 mg/mL
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Supportive validation
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- OriGene (provider)
- Main image
- Experimental details
- WB using Anti-PPAR2 antibody shows detection of PPAR2 protein in a mouse 3T3 whole cell lysate (lane 1 arrowhead). Approximately 20 ug of lysate was loaded onto a 4-20% gradient gel followed by transfer to nitrocellulose. Primary antibody was used at a 1:2,000 dilution. The membrane was washed and reacted with a 1:10,000 dilution of IRDye?800 Conjugated Goat-anti-Rabbit IgG [H&L] MX10. Molecular weight estimation was made by comparison to prestained MW markers indicated at the left (lane M).
- Validation comment
- WB
