BS4217

antibody from Bioworld Technology, Inc

Targeting: CEBPA C/EBP-alpha, CEBP

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

BS4217

Provider

Bioworld Technology, Inc

Product name

C/EBP Ï« (phospho S21) polyclonal antibody

Antibody type

Polyclonal

Antigen

Synthetic phosphopeptide derived from human C/EBP Ï« around the phosphorylation site of Serine 21.

Description

C/EBP Ï« regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. C/EBP Ï« is a basic region/leucine zipper transcription factor selectively expressed during the differentiation of liver, adipose tissue, blood cells and the endocrine pancreas. C/EBP Ï« uses a bipartite structural motif to bind DNA and appears to function exclusively in terminally differentiated, growtharrested cells. In the liver, C/EBP Ï« is a transactivator of several genes, which are regulated by growth hormone. Growth hormone enhances not only the levels of C/EBP Ï« mRNA and protein, but also the DNA-binding activity of C/EBP Ï«. C/EBP Ï« functions as an important transcription factor that regulates different genes, including Prolactin gene expression.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100ul

Concentration

1 mg/ml

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Bioworld Technology, Inc (provider)

Main image

Experimental details

Western blot (WB) analysis of p-C/EBP ¦Á (S21) pAb at 1:500 dilutionLane1:Hela cell lysate treated with EGF(0.1ng/ml,30mins)Lane2:Raw264.7 cell lysate treated with EGF(0.1ng/ml,30mins)Lane3:H9C2 cell lysate treated with EGF(0.1ng/ml,30mins)