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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF-441-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse B7-2/CD86 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse B7-2/CD86 in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing and reducing conditions), less than 15% cross-reactivity with recombinant human B7-2 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 200 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Antigen-loaded ER microsomes from APC induce potent immune responses against viral infection.
Suppression of activation and induction of apoptosis in RAW264.7 cells by amniotic membrane extract.
Sofra V, Mansour S, Liu M, Gao B, Primpidou E, Wang P, Li S
European journal of immunology 2009 Jan;39(1):85-95
European journal of immunology 2009 Jan;39(1):85-95
Suppression of activation and induction of apoptosis in RAW264.7 cells by amniotic membrane extract.
He H, Li W, Chen SY, Zhang S, Chen YT, Hayashida Y, Zhu YT, Tseng SC
Investigative ophthalmology & visual science 2008 Oct;49(10):4468-75
Investigative ophthalmology & visual science 2008 Oct;49(10):4468-75
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- IL-2 Secretion Induced by CH-1 Cell Surface B7-2 and Neutralization by Mouse B7-2/CD86 Antibody. The CH-1 mouse B cell lymphoma cell line, which expresses B7-1 and B7-2 on the cell surface, co-stimulates IL-2 secretion in the Jurkat human acute T cell leukemia cell line in the presence of PHA (10 µg/mL) in a dose-dependent manner (orange line), as measured by the Human IL-2 Quantikine ELISA Kit (Catalog # D2050). Under these conditions, IL-2 secretion elicited by CH-1 cells is neutralized (green line) by increasing concentrations of Goat Anti-Mouse B7-2/CD86 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-441-NA). The ND50 is typically 2-5 µg/mL.
