orb147033

antibody from Biorbyt

Targeting: CAMK2A CAMKA, CaMKIINalpha, CaMKIIα, KIAA0968

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Radioimmunoassay (Recommended by provider)

Antibody Data

Product number

orb147033

Provider

Biorbyt

Product name

CaMKII antibody (ATTO-565)

Antibody type

Monoclonal

Description

Mouse monoclonal to CaMKII (ATTO-565). CaMKII is an important member of calcium/calmodulin- activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling. CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+ auto inhibitory effect and activates the kinase. /calmodulin to its regulatory domain releases its This kinase activation results in auto phosphorylation at threonine 286. The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation. Auto phosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12)..

Reactivity

Rat

Host

Mouse

Isotype

IgG

Antibody clone number

6G9

Vial size

100 μg

Concentration

1 mg/ml

Storage

Store at 2-8°C and -20°C for one year. (Shipped on ice packs)