Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-38238 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Phospho-CaMKII alpha/beta/delta (Thr305) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-CaM Kinase II alpha + beta + delta pThr305 in extracts from NIH-3T3 cells using a Phospho-CaM Kinase II alpha + beta + delta pThr305 polyclonal antibody (Product # PA5-38238).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of SK-N-AS (Lane 1), SK-N-AS treated with Ionomycin (6uM for 24hr) (Lane 2). The blot was probed with Anti-Phospho-CaMKII alpha/beta/delta (Thr305) Polyclonal Antibody (Product # PA5-38238, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 60 kDa band corresponding to Phospho-CaMKII alpha/beta/delta (Thr305) was enhanced in SK-N-AS upon Ionomycin treatment.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-CaM Kinase II alpha + beta + delta pThr305 in paraffin-embedded human brain tissue using a Phospho-CaM Kinase II alpha + beta + delta pThr305 polyclonal antibody (Product # PA5-38238).