Explore
Validate
Learn
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [32]
- Comments [0]
- Validations
- Flow cytometry [2]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MHCD0830 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD8 Monoclonal Antibody (3B5), Pacific Orange™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Our Pacific Orange™ dye is optimally excited by the violet laser, and it is recommended that a 575, 585 or 600 nm band pass filter be used for optimal detection. Pacific Blue™ and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm and 551 nm, respectively, facilitating two-color analysis.
- Antibody clone number
- 3B5
Submitted references Preexisting memory CD4 T cells in naïve individuals confer robust immunity upon hepatitis B vaccination.
Donor Cell Composition and Reactivity Predict Risk of Acute Graft-versus-Host Disease after Allogeneic Hematopoietic Stem Cell Transplantation.
Long-Term Stable Mixed Chimerism after Hematopoietic Stem Cell Transplantation in Patients with Non-Malignant Disease, Shall We Be Tolerant?
Multidimensional Clusters of CD4+ T Cell Dysfunction Are Primarily Associated with the CD4/CD8 Ratio in Chronic HIV Infection.
Variation in Gamma-Globin Expression before and after Induction with Hydroxyurea Associated with BCL11A, KLF1 and TAL1.
Noncytolytic CD8+ Cell Mediated Antiviral Response Represents a Strong Element in the Immune Response of Simian Immunodeficiency Virus-Infected Long-Term Non-Progressing Rhesus Macaques.
Restoration of CD4+ Responses to Copathogens in HIV-Infected Individuals on Antiretroviral Therapy Is Dependent on T Cell Memory Phenotype.
IL-12-secreting CD19-targeted cord blood-derived T cells for the immunotherapy of B-cell acute lymphoblastic leukemia.
CMV-specific T cells generated from naïve T cells recognize atypical epitopes and may be protective in vivo.
A phase II study of bortezomib plus prednisone for initial therapy of chronic graft-versus-host disease.
Comparative analysis of SIV-specific cellular immune responses induced by different vaccine platforms in rhesus macaques.
Phenotype and function of T cells infiltrating visceral metastases from gastrointestinal cancers and melanoma: implications for adoptive cell transfer therapy.
Phase I study of alemtuzumab for therapy of steroid-refractory chronic graft-versus-host disease.
Vitamin B6 and the immunity in kidney transplant recipients.
Expression of integrin α2 receptor in human cord blood CD34+CD38-CD90+ stem cells engrafting long-term in NOD/SCID-IL2Rγ(c) null mice.
Effect of chronic morphine administration on circulating T cell population dynamics in rhesus macaques.
Trichosanthin-stimulated dendritic cells induce a type 2 helper T lymphocyte response through the OX40 ligand.
Bortezomib-based graft-versus-host disease prophylaxis in HLA-mismatched unrelated donor transplantation.
CXCL12-induced monocyte-endothelial interactions promote lymphocyte transmigration across an in vitro blood-brain barrier.
Evaluation of the safety and immunogenicity in rhesus monkeys of a recombinant malaria vaccine for Plasmodium vivax with a synthetic Toll-like receptor 4 agonist formulated in an emulsion.
The stromal cell antigen CD248 (endosialin) is expressed on naive CD8+ human T cells and regulates proliferation.
Interleukin-2 and regulatory T cells in graft-versus-host disease.
Forward light scatter is a simple measure of T-cell activation and proliferation but is not universally suited for doublet discrimination.
Phenotypically and functionally distinct subsets contribute to the expansion of CD56-/CD16+ natural killer cells in HIV infection.
CD4+CD25+Foxp3+ Tregs resolve experimental lung injury in mice and are present in humans with acute lung injury.
HIV-induced changes in T cell signaling pathways.
Flow cytometry identification and characterization of mononuclear cell subsets in the neotropical primate Saimiri sciureus (squirrel monkey).
Screening for T cell-eliciting proteins of Japanese encephalitis virus in a healthy JE-endemic human cohort using recombinant baculovirus-infected insect cell preparations.
Trafficking machinery of NKT cells: shared and differential chemokine receptor expression among V alpha 24(+)V beta 11(+) NKT cell subsets with distinct cytokine-producing capacity.
Cryptomonad algal phycobiliproteins as fluorochromes for extracellular and intracellular antigen detection by flow cytometry.
Expression and coreceptor activity of STRL33/Bonzo on primary peripheral blood lymphocytes.
CK beta-11/macrophage inflammatory protein-3 beta/EBI1-ligand chemokine is an efficacious chemoattractant for T and B cells.
Elias G, Meysman P, Bartholomeus E, De Neuter N, Keersmaekers N, Suls A, Jansens H, Souquette A, De Reu H, Emonds MP, Smits E, Lion E, Thomas PG, Mortier G, Van Damme P, Beutels P, Laukens K, Van Tendeloo V, Ogunjimi B
eLife 2022 Jan 25;11
eLife 2022 Jan 25;11
Donor Cell Composition and Reactivity Predict Risk of Acute Graft-versus-Host Disease after Allogeneic Hematopoietic Stem Cell Transplantation.
Sairafi D, Stikvoort A, Gertow J, Mattsson J, Uhlin M
Journal of immunology research 2016;2016:5601204
Journal of immunology research 2016;2016:5601204
Long-Term Stable Mixed Chimerism after Hematopoietic Stem Cell Transplantation in Patients with Non-Malignant Disease, Shall We Be Tolerant?
Stikvoort A, Sundin M, Uzunel M, Gertow J, Sundberg B, Schaffer M, Mattsson J, Uhlin M
PloS one 2016;11(5):e0154737
PloS one 2016;11(5):e0154737
Multidimensional Clusters of CD4+ T Cell Dysfunction Are Primarily Associated with the CD4/CD8 Ratio in Chronic HIV Infection.
Frederiksen J, Buggert M, Noyan K, Nowak P, Sönnerborg A, Lund O, Karlsson AC
PloS one 2015;10(9):e0137635
PloS one 2015;10(9):e0137635
Variation in Gamma-Globin Expression before and after Induction with Hydroxyurea Associated with BCL11A, KLF1 and TAL1.
Grieco AJ, Billett HH, Green NS, Driscoll MC, Bouhassira EE
PloS one 2015;10(6):e0129431
PloS one 2015;10(6):e0129431
Noncytolytic CD8+ Cell Mediated Antiviral Response Represents a Strong Element in the Immune Response of Simian Immunodeficiency Virus-Infected Long-Term Non-Progressing Rhesus Macaques.
Javed A, Leuchte N, Neumann B, Sopper S, Sauermann U
PloS one 2015;10(11):e0142086
PloS one 2015;10(11):e0142086
Restoration of CD4+ Responses to Copathogens in HIV-Infected Individuals on Antiretroviral Therapy Is Dependent on T Cell Memory Phenotype.
Riou C, Tanko RF, Soares AP, Masson L, Werner L, Garrett NJ, Samsunder N, Karim QA, Karim SSA, Burgers WA
Journal of immunology (Baltimore, Md. : 1950) 2015 Sep 1;195(5):2273-2281
Journal of immunology (Baltimore, Md. : 1950) 2015 Sep 1;195(5):2273-2281
IL-12-secreting CD19-targeted cord blood-derived T cells for the immunotherapy of B-cell acute lymphoblastic leukemia.
Pegram HJ, Purdon TJ, van Leeuwen DG, Curran KJ, Giralt SA, Barker JN, Brentjens RJ
Leukemia 2015 Feb;29(2):415-22
Leukemia 2015 Feb;29(2):415-22
CMV-specific T cells generated from naïve T cells recognize atypical epitopes and may be protective in vivo.
Hanley PJ, Melenhorst JJ, Nikiforow S, Scheinberg P, Blaney JW, Demmler-Harrison G, Cruz CR, Lam S, Krance RA, Leung KS, Martinez CA, Liu H, Douek DC, Heslop HE, Rooney CM, Shpall EJ, Barrett AJ, Rodgers JR, Bollard CM
Science translational medicine 2015 Apr 29;7(285):285ra63
Science translational medicine 2015 Apr 29;7(285):285ra63
A phase II study of bortezomib plus prednisone for initial therapy of chronic graft-versus-host disease.
Herrera AF, Kim HT, Bindra B, Jones KT, Alyea EP 3rd, Armand P, Cutler CS, Ho VT, Nikiforow S, Blazar BR, Ritz J, Antin JH, Soiffer RJ, Koreth J
Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation 2014 Nov;20(11):1737-43
Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation 2014 Nov;20(11):1737-43
Comparative analysis of SIV-specific cellular immune responses induced by different vaccine platforms in rhesus macaques.
Valentin A, McKinnon K, Li J, Rosati M, Kulkarni V, Pilkington GR, Bear J, Alicea C, Vargas-Inchaustegui DA, Jean Patterson L, Pegu P, Liyanage NPM, Gordon SN, Vaccari M, Wang Y, Hogg AE, Frey B, Sui Y, Reed SG, Sardesai NY, Berzofsky JA, Franchini G, Robert-Guroff M, Felber BK, Pavlakis GN
Clinical immunology (Orlando, Fla.) 2014 Nov;155(1):91-107
Clinical immunology (Orlando, Fla.) 2014 Nov;155(1):91-107
Phenotype and function of T cells infiltrating visceral metastases from gastrointestinal cancers and melanoma: implications for adoptive cell transfer therapy.
Turcotte S, Gros A, Hogan K, Tran E, Hinrichs CS, Wunderlich JR, Dudley ME, Rosenberg SA
Journal of immunology (Baltimore, Md. : 1950) 2013 Sep 1;191(5):2217-25
Journal of immunology (Baltimore, Md. : 1950) 2013 Sep 1;191(5):2217-25
Phase I study of alemtuzumab for therapy of steroid-refractory chronic graft-versus-host disease.
Nikiforow S, Kim HT, Bindra B, McDonough S, Glotzbecker B, Armand P, Koreth J, Ho VT, Alyea EP 3rd, Blazar BR, Ritz J, Soiffer RJ, Antin JH, Cutler CS
Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation 2013 May;19(5):804-11
Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation 2013 May;19(5):804-11
Vitamin B6 and the immunity in kidney transplant recipients.
Jankowska M, Marszałł M, Dębska-Ślizień A, Carrero JJ, Lindholm B, Czarnowski W, Rutkowski B, Trzonkowski P
Journal of renal nutrition : the official journal of the Council on Renal Nutrition of the National Kidney Foundation 2013 Jan;23(1):57-64
Journal of renal nutrition : the official journal of the Council on Renal Nutrition of the National Kidney Foundation 2013 Jan;23(1):57-64
Expression of integrin α2 receptor in human cord blood CD34+CD38-CD90+ stem cells engrafting long-term in NOD/SCID-IL2Rγ(c) null mice.
Wong WM, Sigvardsson M, Åstrand-Grundström I, Hogge D, Larsson J, Qian H, Ekblom M
Stem cells (Dayton, Ohio) 2013 Feb;31(2):360-71
Stem cells (Dayton, Ohio) 2013 Feb;31(2):360-71
Effect of chronic morphine administration on circulating T cell population dynamics in rhesus macaques.
Cornwell WD, Lewis MG, Fan X, Rappaport J, Rogers TJ
Journal of neuroimmunology 2013 Dec 15;265(1-2):43-50
Journal of neuroimmunology 2013 Dec 15;265(1-2):43-50
Trichosanthin-stimulated dendritic cells induce a type 2 helper T lymphocyte response through the OX40 ligand.
Yan R, Zhong W, Zhu Y, Zhang X
Journal of investigational allergology & clinical immunology 2012;22(7):491-500
Journal of investigational allergology & clinical immunology 2012;22(7):491-500
Bortezomib-based graft-versus-host disease prophylaxis in HLA-mismatched unrelated donor transplantation.
Koreth J, Stevenson KE, Kim HT, McDonough SM, Bindra B, Armand P, Ho VT, Cutler C, Blazar BR, Antin JH, Soiffer RJ, Ritz J, Alyea EP 3rd
Journal of clinical oncology : official journal of the American Society of Clinical Oncology 2012 Sep 10;30(26):3202-8
Journal of clinical oncology : official journal of the American Society of Clinical Oncology 2012 Sep 10;30(26):3202-8
CXCL12-induced monocyte-endothelial interactions promote lymphocyte transmigration across an in vitro blood-brain barrier.
Man S, Tucky B, Cotleur A, Drazba J, Takeshita Y, Ransohoff RM
Science translational medicine 2012 Feb 1;4(119):119ra14
Science translational medicine 2012 Feb 1;4(119):119ra14
Evaluation of the safety and immunogenicity in rhesus monkeys of a recombinant malaria vaccine for Plasmodium vivax with a synthetic Toll-like receptor 4 agonist formulated in an emulsion.
Lumsden JM, Pichyangkul S, Srichairatanakul U, Yongvanitchit K, Limsalakpetch A, Nurmukhambetova S, Klein J, Bertholet S, Vedvick TS, Reed SG, Sattabongkot J, Bennett JW, Polhemus ME, Ockenhouse CF, Howard RF, Yadava A
Infection and immunity 2011 Sep;79(9):3492-500
Infection and immunity 2011 Sep;79(9):3492-500
The stromal cell antigen CD248 (endosialin) is expressed on naive CD8+ human T cells and regulates proliferation.
Hardie DL, Baldwin MJ, Naylor A, Haworth OJ, Hou TZ, Lax S, Curnow SJ, Willcox N, MacFadyen J, Isacke CM, Buckley CD
Immunology 2011 Jul;133(3):288-95
Immunology 2011 Jul;133(3):288-95
Interleukin-2 and regulatory T cells in graft-versus-host disease.
Koreth J, Matsuoka K, Kim HT, McDonough SM, Bindra B, Alyea EP 3rd, Armand P, Cutler C, Ho VT, Treister NS, Bienfang DC, Prasad S, Tzachanis D, Joyce RM, Avigan DE, Antin JH, Ritz J, Soiffer RJ
The New England journal of medicine 2011 Dec 1;365(22):2055-66
The New England journal of medicine 2011 Dec 1;365(22):2055-66
Forward light scatter is a simple measure of T-cell activation and proliferation but is not universally suited for doublet discrimination.
Böhmer RM, Bandala-Sanchez E, Harrison LC
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2011 Aug;79(8):646-52
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2011 Aug;79(8):646-52
Phenotypically and functionally distinct subsets contribute to the expansion of CD56-/CD16+ natural killer cells in HIV infection.
Hong HS, Eberhard JM, Keudel P, Bollmann BA, Ahmad F, Ballmaier M, Bhatnagar N, Zielinska-Skowronek M, Schmidt RE, Meyer-Olson D
AIDS (London, England) 2010 Jul 31;24(12):1823-34
AIDS (London, England) 2010 Jul 31;24(12):1823-34
CD4+CD25+Foxp3+ Tregs resolve experimental lung injury in mice and are present in humans with acute lung injury.
D'Alessio FR, Tsushima K, Aggarwal NR, West EE, Willett MH, Britos MF, Pipeling MR, Brower RG, Tuder RM, McDyer JF, King LS
The Journal of clinical investigation 2009 Oct;119(10):2898-913
The Journal of clinical investigation 2009 Oct;119(10):2898-913
HIV-induced changes in T cell signaling pathways.
Schweneker M, Favre D, Martin JN, Deeks SG, McCune JM
Journal of immunology (Baltimore, Md. : 1950) 2008 May 15;180(10):6490-500
Journal of immunology (Baltimore, Md. : 1950) 2008 May 15;180(10):6490-500
Flow cytometry identification and characterization of mononuclear cell subsets in the neotropical primate Saimiri sciureus (squirrel monkey).
Contamin H, Loizon S, Bourreau E, Michel JC, Garraud O, Mercereau-Puijalon O, Behr C
Journal of immunological methods 2005 Feb;297(1-2):61-71
Journal of immunological methods 2005 Feb;297(1-2):61-71
Screening for T cell-eliciting proteins of Japanese encephalitis virus in a healthy JE-endemic human cohort using recombinant baculovirus-infected insect cell preparations.
Kumar P, Uchil PD, Sulochana P, Nirmala G, Chandrashekar R, Haridattatreya M, Satchidanandam V
Archives of virology 2003 Aug;148(8):1569-91
Archives of virology 2003 Aug;148(8):1569-91
Trafficking machinery of NKT cells: shared and differential chemokine receptor expression among V alpha 24(+)V beta 11(+) NKT cell subsets with distinct cytokine-producing capacity.
Kim CH, Johnston B, Butcher EC
Blood 2002 Jul 1;100(1):11-6
Blood 2002 Jul 1;100(1):11-6
Cryptomonad algal phycobiliproteins as fluorochromes for extracellular and intracellular antigen detection by flow cytometry.
Telford WG, Moss MW, Morseman JP, Allnutt FC
Cytometry 2001 May 1;44(1):16-23
Cytometry 2001 May 1;44(1):16-23
Expression and coreceptor activity of STRL33/Bonzo on primary peripheral blood lymphocytes.
Sharron M, Pöhlmann S, Price K, Lolis E, Tsang M, Kirchhoff F, Doms RW, Lee B
Blood 2000 Jul 1;96(1):41-9
Blood 2000 Jul 1;96(1):41-9
CK beta-11/macrophage inflammatory protein-3 beta/EBI1-ligand chemokine is an efficacious chemoattractant for T and B cells.
Kim CH, Pelus LM, White JR, Applebaum E, Johanson K, Broxmeyer HE
Journal of immunology (Baltimore, Md. : 1950) 1998 Mar 1;160(5):2418-24
Journal of immunology (Baltimore, Md. : 1950) 1998 Mar 1;160(5):2418-24
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human peripheral blood lymphocytes were stained using Pacific Orange-8 fluorochrome conjugates of anti-uman CD8 monoclonal antibody (clone 3B5). The negative control profiles represent unstained cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis using a CD8 monoclonal antibody (Product # MHCD0830).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
