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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
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- Product number
- MA1-23200 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- POLD1 Monoclonal Antibody (607)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: 293T, HeLa, BCL-1.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 607
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of POLD1 was performed by separating 30 µg of whole cell extract by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a POLD1 Monoclonal Antibody (607) (Product # MA1-23200) at a dilution of 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using POLD1 Monoclonal Antibody (607) (Product # MA1-23200). Sample (30 µg of whole cell lysate). Lane A: BCL-1. 5% SDS PAGE. POLD1 Monoclonal Antibody (607) (Product # MA1-23200) diluted at 1:1,000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using POLD1 Monoclonal Antibody (607) (Product # MA1-23200). Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with POLD1 Monoclonal Antibody (607) (Product # MA1-23200) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using POLD1 Monoclonal Antibody (607) (Product # MA1-23200). Detection of human DNA polymerase delta by using anti-pol delta monoclonal antibody [607] POLD1 Monoclonal Antibody (607) (Product # MA1-23200) in HeLa whole cell extract. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
