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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [3]
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Validation data
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- Product number
- TA504365 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA504365, RRID:AB_11124815
- Product name
- ERCC1 mouse monoclonal antibody, clone OTI1C10 (formerly 1C10)
- Antibody type
- Monoclonal
- Description
- ERCC1 mouse monoclonal antibody, clone OTI1C10 (formerly 1C10)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- ERCC1
- Isotype
- IgG
- Antibody clone number
- OTI1C10
- Vial size
- 100 µl
- Concentration
- 0.73 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ERCC1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (RC200478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-ERCC1 mouse monoclonal antibody (TA504365) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC1(RC200478).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-ERCC1 antibody(TA504365),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC200478 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ERCC1 antibody(TA504365), and then analyzed by flow cytometry.
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-ERCC1 antibody(TA504365),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
