LS-C312273

antibody from LSBio

Targeting: GAD1 GAD

Western blot

Immunohistochemistry

Antibody data

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Validation data

Product number: LS-C312273 - Provider product page
Provider: LSBio
Product name: GAD1 / GAD67 Antibody (aa10-24) LS-C312273
Antibody type: Polyclonal
Description: Immunogen affinity purified
Reactivity: Human, Mouse, Rat, Bovine, Guinea Pig, Rabbit, Simian
Host: Rabbit
Storage: At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

GAD1 protein structure - LS-C312273 shown in red.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: ICC analysis of GAD67 using anti-GAD67 antibody. GAD67 was detected in immunocytochemical section of A431 cells. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 3µg/ml rabbit anti-GAD67 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: ICC analysis of GAD67 using anti-GAD67 antibody. GAD67 was detected in immunocytochemical section of A431 cells. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 3µg/ml rabbit anti-GAD67 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: ICC analysis of GAD67 using anti-GAD67 antibody. GAD67 was detected in immunocytochemical section of A431 cells. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 3µg/ml rabbit anti-GAD67 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.