Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- AF871 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IGFBP-1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human IGFBP-1 in direct ELISAs and Western blots. In these formats, less than 2% cross-reactivity with recombinant human (rh) IGFBP-2, rhIGFBP-3, rhIGFBP-4, and rhIGFBP-5 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references The effect of cinnamon on polycystic ovary syndrome in a mouse model.
The role of matrix metalloproteinase-7 in redefining the gastric microenvironment in response to Helicobacter pylori.
Dou L, Zheng Y, Li L, Gui X, Chen Y, Yu M, Guo Y
Reproductive biology and endocrinology : RB&E 2018 Oct 19;16(1):99
Reproductive biology and endocrinology : RB&E 2018 Oct 19;16(1):99
The role of matrix metalloproteinase-7 in redefining the gastric microenvironment in response to Helicobacter pylori.
McCaig C, Duval C, Hemers E, Steele I, Pritchard DM, Przemeck S, Dimaline R, Ahmed S, Bodger K, Kerrigan DD, Wang TC, Dockray GJ, Varro A
Gastroenterology 2006 May;130(6):1754-63
Gastroenterology 2006 May;130(6):1754-63
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human IGFBP-1 by Simple Western<SUP abp="261">TM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for IGFBP-1 at approximately 39 kDa (as indicated) using 50 µg/mL of Goat Anti-Human IGFBP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF871) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human IGFBP-1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IGFBP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF871) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IGFBP-1 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.