MAB2001-100

antibody from Novus Biologicals

Targeting: IL1A IL-1A, IL1, IL1-ALPHA, IL1F1

Flow cytometry (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB2001-100

Provider

Novus Biologicals

Product name

Mouse Monoclonal IL-1 alpha/IL-1F1 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from ascites. Detects human IL-1 alpha/IL-1F1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse IL-1 alpha , recombinant rat IL-1 alpha , recombinant cotton rat IL-1 alpha , recombinant human (rh) IL-1 beta or rhIL-18 is observed.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Cell Proliferation Induced by IL-1 alpha/IL-1F1 and Neutralization by Human IL-1 alpha/IL-1F1 Antibody. Recombinant Human IL-1 alpha/IL-1F1 (Catalog # 200-LA) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-1 alpha/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Human IL-1 alpha/IL-1F1 Membrane Form Monoclonal Antibody (Catalog # MAB2001). The ND₅₀ is typically 5-20 µg/mL in the presence of concanavalin A (1.25 µg/mL).