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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF500 - Provider product page
- Provider
- R&D Systems
- Product name
- Rat IL-1 alpha/IL-1F1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects rat IL-1 alpha/IL-1F1 in direct ELISAs and Western blots. In direct ELISAs, less than 50% cross-reactivity with recombinant mouse IL-1 alpha/IL-1F1 and recombinant cotton rat IL-1 alpha/IL-1F1 is observed, and less than 15% cross-reactivity with recombinant human IL-1 alpha/IL-1F1 and recombinant porcine IL-1 alpha/IL-1F1 is observed.
- Reactivity
- Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P16598- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Regulation of interleukin 1alpha, activin and inhibin by lipopolysaccharide in Sertoli cells from prepubertal rats.
Interleukin-1 mediates the anorexic and febrile actions of galanin-like Peptide.
Winnall WR, Okuma Y, Saito K, Muir JA, Hedger MP
Molecular and cellular endocrinology 2009 Aug 13;307(1-2):169-75
Molecular and cellular endocrinology 2009 Aug 13;307(1-2):169-75
Interleukin-1 mediates the anorexic and febrile actions of galanin-like Peptide.
Man PS, Lawrence CB
Endocrinology 2008 Nov;149(11):5791-802
Endocrinology 2008 Nov;149(11):5791-802
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by IL-1 alpha/IL-1F1 and Neutralization by Rat IL-1 alpha/IL-1F1 Antibody. Recombinant Rat IL-1 alpha/IL-1F1 (Catalog # 500-RL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-1 alpha/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IL-1 alpha/IL-1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF500). The ND50 is typically 0.003-0.009 µg/mL in the presence of concanavalin A (1.25 µg/mL).
