ABIN102983

antibody from antibodies-online

Targeting: IL1A IL-1A, IL1, IL1-ALPHA, IL1F1

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

ABIN102983

Provider

antibodies-online

Proper citation

Antibodies-Online Cat#ABIN102983, RRID:AB_10778810

Product name

anti-Interleukin 1 alpha (IL1A) (Cleavage Site) antibody

Antibody type

Polyclonal

Description

This is an IgG preparation of whole rabbit serum purified by DEAE fractionation.  This antibody is primarily directed against the 17,000 MW human IL-1a and is useful in determining its presence in various assays.  In general, this antibody also detects primate IL-1a in the same formats using similar dilutions.  The antiserum does not recognize human IL-1b or Mouse or Rabbit IL-1a.  In ELISA formats and other immunoreactive assays, this antibody will recognize both the mature 17,000 MW IL-1a as well as the 31,000 MW IL-1a precursor in either non-denatured (native) or denatured samples.  Unlike the IL-1b precursor, the native precursor of IL-1a is recognized by the antibody produced to the 17,000 MW form.   The 31,000 precursor of IL-1a is biologically active and is found primarily intracellularly.  The precursor of IL-1a, unlike that of IL-1b, is biologically active when applied to cells and is thought to have a role as a functional molecule intracellularly and can be found constitutively expressed in various cell.  This antibody is also useful for neutralization of human and primate IL-1a activity in bioassays.  It does not neutralize the biological activity IL-1b.  It does not neutralize the biological activity of mouse, rat or rabbit IL-1a.  It will neutralize primate IL-a.  For neutralization, it is recommended to incubate the sample with a 1:100 dilution of the antibody for at least 4 hours before being tested.  A control of similarly diluted normal rabbit IgG is recommended.  This antibody can be used for FACS analysis.  Caution should be exhibited as the F( c) domain of the rabbit IgG molecule may interact with cells non-specifically.

Reactivity

Human

Host

Rabbit

Epitope

Cleavage Site

Isotype

IgG

Vial size

1 mg

Storage

4°C,-20°C