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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- AF6844 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-4R alpha Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human IL-4 R alpha in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse IL-4 R is observed, and less than 1% cross-reactivity with recombinant human (rh) IL-5 R alpha , rhIL-5 R beta , rhIL-9 R, rhIL-13 R alpha 1, and rhIL-13 R alpha 2 is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P24394- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- IL-4 R alpha in Human PBMCs. IL-4 R alpha was detected in immersion fixed unstimulated human peripheral blood mononuclear cells (PBMCs) using Sheep Anti-Human IL-4 R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6844) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
