Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- NB200-326 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB200-326, RRID:AB_10003198
- Product name
- Rabbit Polyclonal LIS1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Human mutations in NDE1 cause extreme microcephaly with lissencephaly [corrected].
Functional interplay between LIS1, NDE1 and NDEL1 in dynein-dependent organelle positioning.
APC and EB1 function together in mitosis to regulate spindle dynamics and chromosome alignment.
Alkuraya FS, Cai X, Emery C, Mochida GH, Al-Dosari MS, Felie JM, Hill RS, Barry BJ, Partlow JN, Gascon GG, Kentab A, Jan M, Shaheen R, Feng Y, Walsh CA
American journal of human genetics 2011 May 13;88(5):536-47
American journal of human genetics 2011 May 13;88(5):536-47
Functional interplay between LIS1, NDE1 and NDEL1 in dynein-dependent organelle positioning.
Lam C, Vergnolle MA, Thorpe L, Woodman PG, Allan VJ
Journal of cell science 2010 Jan 15;123(Pt 2):202-12
Journal of cell science 2010 Jan 15;123(Pt 2):202-12
APC and EB1 function together in mitosis to regulate spindle dynamics and chromosome alignment.
Green RA, Wollman R, Kaplan KB
Molecular biology of the cell 2005 Oct;16(10):4609-22
Molecular biology of the cell 2005 Oct;16(10):4609-22
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: LIS1 Antibody [NB200-326] - Detection of Human, Mouse and Rat LIS-1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, K562, mouse TCMK-1, and rat C6 cells. Antibodies: Affinity purified rabbit anti-LIS-1 antibody NB200-326 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: LIS1 Antibody [NB200-326] - Whole cell lysate (50 ug) from TF-1 (erythroleukemia) cells. Antibody used at the indicated concentrations.