Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- PA5-47145 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LRP6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse LRP-6 is observed, and less than 1% cross-reactivity with recombinant human (rh) LRP-1, rhLRP-4, and rhLRP-5 is observed.
- Concentration
- 0.2 mg/mL
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis from lysates of 293T human embryonic kidney cell line either mock transfected or transfected with human LRP-6. PVDF Membrane was probed with 1 µg/mL of Goat Anti-human LRP-6 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47145) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for LRP-6 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LRP6 in 293T human embryonic kidney cell line either mock transfected or transfected with human LRP‚6. Samples were incubated in LRP6 polyclonal antibody (Product # PA5-47145) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for LRP‚6 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions.