Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- MAB59941 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Neuropilin-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse Neuropilin-1 in ELISAs.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P97333
- Isotype
- IgG
- Antibody clone number
- 761704
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references CD45Rb-low effector T cells require IL-4 to induce IL-10 in FoxP3 Tregs and to protect mice from inflammation.
The vimentin intermediate filament network restrains regulatory T cell suppression of graft-versus-host disease.
Stability and function of regulatory T cells is maintained by a neuropilin-1-semaphorin-4a axis.
Jones MB, Alvarez CA, Johnson JL, Zhou JY, Morris N, Cobb BA
PloS one 2019;14(5):e0216893
PloS one 2019;14(5):e0216893
The vimentin intermediate filament network restrains regulatory T cell suppression of graft-versus-host disease.
McDonald-Hyman C, Muller JT, Loschi M, Thangavelu G, Saha A, Kumari S, Reichenbach DK, Smith MJ, Zhang G, Koehn BH, Lin J, Mitchell JS, Fife BT, Panoskaltsis-Mortari A, Feser CJ, Kirchmeier AK, Osborn MJ, Hippen KL, Kelekar A, Serody JS, Turka LA, Munn DH, Chi H, Neubert TA, Dustin ML, Blazar BR
The Journal of clinical investigation 2018 Oct 1;128(10):4604-4621
The Journal of clinical investigation 2018 Oct 1;128(10):4604-4621
Stability and function of regulatory T cells is maintained by a neuropilin-1-semaphorin-4a axis.
Delgoffe GM, Woo SR, Turnis ME, Gravano DM, Guy C, Overacre AE, Bettini ML, Vogel P, Finkelstein D, Bonnevier J, Workman CJ, Vignali DA
Nature 2013 Sep 12;501(7466):252-6
Nature 2013 Sep 12;501(7466):252-6
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Neuropilin-1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB554A) and either (A) Rat Anti-Mouse Neuropilin-1 Monoclonal Antibody (Catalog # MAB59941) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).