H00008550-D01P

antibody from Abnova Corporation

Targeting: MAPKAPK5 MK5, PRAK

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00008550-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00008550-D01P, RRID:AB_1577031

Product name

MAPKAPK5 purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human MAPKAPK5 protein.

Antigen sequence

MSEESDMDKAIKETSILEEYSINWTQKLGAGISGP
VRVCVKKSTQERFALKILLDRPKARNEVRLHMMCA
THPNIVQIIEVFANSVQFPHESSPRARLLIVMEMM
EGGELFHRISQHRHFTEKQASQVTKQIALALRHCH
LLNIAHRDLKPENLLFKDNSLDAPVKLCDFGFAKI
DQGDLMTPQFTPYYVAPQVLEAQRRHQKEKSGIIP
TSPTPYTYNKSCDLWSLGVIIYVMLCGYPPFYSKH
HSRTIPKDMRRKIMTGSFEFPEEEWSQISEMAKDV
VRKLLKVKPEERLTIEGVLDHPWLNSTEALDNVLP
SAQLMMDKAVVAGIQQAHAEQLANMRIQDLKVSLK
PLHSVNNPILRKRKLLGTKPKDSVYIHDHENGAED
SNVALEKLRDVIAQCILPQAGENEDEKLNEVMQEA
WKYNRECKLLRDTLQSFSWNGRGFTDKVDRLKLAE
IVKQVIEEQTTSHESQ


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of MAPKAPK5 expression in transfected 293T cell line (H00008550-T02) by MAPKAPK5 MaxPab polyclonal antibody.Lane 1: MAPKAPK5 transfected lysate(54.00 KDa).Lane 2: Non-transfected lysate.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between MAPKAPK5 and EIF4EBP1. HeLa cells were stained with anti-MAPKAPK5 rabbit purified polyclonal 1:1200 and anti-EIF4EBP1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)