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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AB-255-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human SCF/c-kit Ligand Antibody
- Antibody type
- Polyclonal
- Description
- Protein A or G purified. Detects human SCF/c-kit Ligand in direct ELISAs and Western blots. In direct ELISA, approximately 45% cross-reactivity with recombinant mouse SCF is observed, approximately 30% cross-reactivity with recombinant canine SCF, recombinant rat SCF, and recombinant feline SCF is observed. It will also neutralize the biological activity of recombinant mouse SCF at a 10 fold higher IgG concentration.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P21583- Isotype
- IgG
- Vial size
- 1 mg
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Human peripheral blood eosinophils express stem cell factor.
Human peripheral blood eosinophils express stem cell factor.
Hartman M, Piliponsky AM, Temkin V, Levi-Schaffer F
Blood 2001 Feb 15;97(4):1086-91
Blood 2001 Feb 15;97(4):1086-91
Human peripheral blood eosinophils express stem cell factor.
Hartman M, Piliponsky AM, Temkin V, Levi-Schaffer F
Blood 2001 Feb 15;97(4):1086-91
Blood 2001 Feb 15;97(4):1086-91
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by SCF/c-kit Ligand and Neutralization by Human SCF/c-kit Ligand Antibody. Recombinant Human SCF/c-kit Ligand (Catalog # 255-SC) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human SCF/c-kit Ligand (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human SCF/c-kit Ligand Polyclonal Antibody (Catalog # AB-255-NA). The ND50 is typically 0.4-2 µg/mL.
