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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Other assay [1]
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- Product number
- MA5-15509 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TrkA Monoclonal Antibody (6B2)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15509 targets TrkA in IF and WB applications and shows reactivity with Human samples.
- Antibody clone number
- 6B2
- Concentration
- Conc. Not Determined
Submitted references Control of mechanical pain hypersensitivity in mice through ligand-targeted photoablation of TrkB-positive sensory neurons.
Dhandapani R, Arokiaraj CM, Taberner FJ, Pacifico P, Raja S, Nocchi L, Portulano C, Franciosa F, Maffei M, Hussain AF, de Castro Reis F, Reymond L, Perlas E, Garcovich S, Barth S, Johnsson K, Lechner SG, Heppenstall PA
Nature communications 2018 Apr 24;9(1):1640
Nature communications 2018 Apr 24;9(1):1640
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TrkA using TrkA monoclonal antibody (Product # MA5-15509) in extracellular domain of human TrkA (aa33-423).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of PC-12 cells using TrkA monoclonal antibody (Product # MA5-15509) (Green). Blue: DRAQ5 fluorescent DNA dye.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of PC-12 cells using TrkA monoclonal antibody (Product # MA5-15509) (Green). Blue: DRAQ5 fluorescent DNA dye.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of PC-12 cells using TrkA monoclonal antibody (Product # MA5-15509) (Green). Blue: DRAQ5 fluorescent DNA dye.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 1 TrkB-positive sensory neurons are putative mechanoreceptors. a - d Double immunofluorescence of DRG sections from TrkB CreERT2 ::Rosa26 RFP mice with a NF200, b Ret, visualized using TrkB CreERT2 ::Rosa26 RFP ::Ret EGFP triple transgenic mice, c IB4, d quantification of staining on mouse DRG sections; TrkB + cells account for ~10% of all DRG neurons and all co-express NF200 or NF200 + Ret eGFP , while they are negative for IB4, CGRP, and TH. e - h Double immunofluorescence of human DRG sections stained with antibodies against TrkB and e NF200, f Ret, g TrkA. h Size distribution for human DRG neurons expressing TrkB, NF200, and TrkA. i Section through the lumbar spinal cord of TrkB CreERT2 ::Avil mCherry mice stained with IB4. j TrkB + lanceolate endings in a section of the back hairy skin of TrkB CreERT2 ::Rosa26 SnapCaaX labeled with Snap Cell TMRstar (red), NF200 (green), and DAPI (blue). k Section from the glabrous skin of TrkB CreERT2 ::Rosa26 ChR2YFP (red) stained with anti-S100 a marker for Meissner's corpuscles (green) and DAPI (blue) showing TrkB + innervation. l Section from human glabrous skin stained with antibodies against TrkB (red) and NF200 (green), and DAPI (blue). Scale bars, a - c and i 50 mum, e - g and j - l 40 mum.
