Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB110-90616 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB110-90616, RRID:AB_1218285
- Product name
- Mouse Monoclonal gamma Tubulin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. The TU-32 recognizes an epitope (amino acids 434-449 in human) within C-terminus of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC).
- Reactivity
- Human, Porcine
- Host
- Mouse
- Antigen sequence
The epitope was located in the amin
oacid sequence PDYISW (aa441-446 in
human), which is identical for gam
ma-tubulin 1 and gamma-tubulin 2.- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Direct effects of phenformin on metabolism/bioenergetics and viability of SH-SY5Y neuroblastoma cells.
Bioenergetics of acquired cisplatin resistant H1299 non-small cell lung cancer and P31 mesothelioma cells.
Geoghegan F, Chadderton N, Farrar GJ, Zisterer DM, Porter RK
Oncology letters 2017 Nov;14(5):6298-6306
Oncology letters 2017 Nov;14(5):6298-6306
Bioenergetics of acquired cisplatin resistant H1299 non-small cell lung cancer and P31 mesothelioma cells.
Geoghegan F, Buckland RJ, Rogers ET, Khalifa K, O'Connor EB, Rooney MF, Behnam-Motlagh P, Nilsson TK, Grankvist K, Porter RK
Oncotarget 2017 Nov 7;8(55):94711-94725
Oncotarget 2017 Nov 7;8(55):94711-94725
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: gamma Tubulin Antibody (TU-32) [NB110-90616] - Analysis of gamma-Tubulin in porcine brain lysate using NBP1-90616.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: gamma Tubulin Antibody (TU-32) [NB110-90616] - Differential reactivity of monoclonal antibodies to gamma-tubulin with human gamma-tubulin isotypes. (A) Immunoblots of total cell lysates from SH-SY5Y cells, expressing TagRFP-tagged human gamma-tubulin 1 (gamma-Tb1) or gamma-tubulin 2 (gamma-Tb2), probed with Abs to gamma-tubulin (TU-30, TU-32), TagRFP (RFP) and GAPDH. In control samples, only secondary anti-mouse Ab was applied. (B) Immunoblots of immobilized GST-tagged human C-terminal regions (a.a. 362-451) of gamma-Tb1 or gamma-Tb2 probed with Abs to gamma-tubulin (TU-30, TU-32) and GST. In control samples, only secondary anti-mouse Ab was applied.
