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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [4]
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- Product number
- PA5-51654 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SIX1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: CFKEKSRGVL REWYAHNPYP SPREKRELAE ATGLTTTQVS NWFKNRRQRD RAAEAKEREN TENNNSSSNK QNQLSPLEGG KPLMSSSEEE FSPPQSPDQN SVLLLQGNMG HARSSNYSLP GLTASQPSHG LQTHQHQLQD S
- Concentration
- 0.4 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SIX1 in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using a SIX1 Polyclonal Antibody (Product # PA5-51654). Remaining relative intensity is presented. Loading control: Anti-PPIB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SIX1 in mouse cell line NIH-3T3 using a SIX1 Polyclonal Antibody (Product # PA5-51654).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SIX1 in control (vector only transfected HEK293T lysate) and SIX1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a SIX1 Polyclonal Antibody (Product # PA5-51654).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of SIX1 in human cell line U-251 MG shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a SIX1 Polyclonal Antibody (Product # PA5-51654).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of SIX1 in human cell line A-431 using a SIX1 Polyclonal Antibody (Product # PA5-51654) shows localization to nucleus and nucleoli.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SIX1 in human skeletal muscle using SIX1 Polyclonal Antibody (Product # PA5-51654) shows strong nuclear positivity in striated muscle fibers.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SIX1 in human breast cancer using a SIX1 Polyclonal Antibody (Product # PA5-51654) shows weak to moderate nuclear positivity in tumor cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SIX1 in human salivary gland using a SIX1 Polyclonal Antibody (Product # PA5-51654) shows moderate to strong nuclear positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SIX1 in human liver using a SIX1 Polyclonal Antibody (Product # PA5-51654) shows no positivity in hepatocytes as expected.
