702307

antibody from Invitrogen Antibodies

Targeting: CCL21 6Ckine, CKb9, ECL, exodus-2, SCYA21, SLC, TCA4

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

702307

Provider

Invitrogen Antibodies

Product name

CCL21 Recombinant Rabbit Monoclonal Antibody (6H4L18)

Antibody type

Monoclonal

Antigen

Other

Description

This antibody is predicted to react with Monkey, Dog, Cat and Horse

Antibody clone number

6H4L18

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on Membrane extracts (30 µg lysate) of MCF-7 (Lane 1), T-47D (Lane 2), MDA-MB-231 (Lane 3), Caco-2 (Lane 4), HCT 116 (Lane 5), SW480 (Lane 6) and HT-29 (Lane 7). The blots were probed with Anti-CCL21 Recombinant Rabbit Monoclonal Antibody (Product # 702307, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 15 kDa band corresponding to CCL21 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of endogenous CCL21 was performed on MCF7 cells labeled with ABfinity™ Anti-CCL21 Recombinant Rabbit Monoclonal Antibody (Product # 702307, 5 ug/ 1M cells) or with rabbit isotype control at 0.5 ug/ml and detected with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, (Alexa Fluor® 488 conjugate, Product # A27034, 0.4 ug/ml, 1:2500) as represented by the red and pink histograms respectively. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control. A representative of 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer (4468770).