PA5-27879

antibody from Invitrogen Antibodies

Targeting: ACTR2 ARP2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27879

Provider

Invitrogen Antibodies

Product name

Arp2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: H1299, HeLa, HepG2, Molt-4, Raji, NIH-3T3.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Arp2 using 30 µg of NIH-3T3 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an Arp2 polyclonal antibody (Product # PA5-27879) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Arp2 was performed by separating 50 µg of various tissue extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Arp2 Polyclonal Antibody (Product # PA5-27879) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Arp2 Polyclonal Antibody (Product # PA5-27879). Sample (30 µg of whole cell lysate). Lane A: H1299. 10% SDS PAGE. Arp2 Polyclonal Antibody (Product # PA5-27879) diluted at 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of Arp2 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Arp2 Polyclonal Antibody (Product # PA5-27879) diluted at 1:100. Red: phalloidin, a cytoskeleton marker. Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Arp2 Polyclonal Antibody detects Arp2 protein at cytosol on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. Arp2 Polyclonal Antibody (Product # PA5-27879) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human heart, using Arp2 (Product # PA5-27879) antibody (10 µg/mL). Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.