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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- MA5-26818 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CTGF Monoclonal Antibody (OTI4B12)
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI4B12
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CTGF in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with CTGF (Product # MA5-26818) monoclonal antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot was performed using Anti-CTGF Monoclonal Antibody (OTI4B12) (Product # MA5-26818) and a 38 kDa band corresponding to Connective tissue growth factor was observed across in HUVEC and HepG2 upon PTI treatment, but not in HEK 293 upon PTI treatment. Whole cell extracts (30 µg lysate) of HUVEC (Lane 1), HUVEC (Protein Transport Inhibitor Cocktail (500X), 1X, 4 hours)(Lane 2), HEK-293 (Lane 3), HEK-293 (Protein Transport Inhibitor Cocktail (500X), 1X, 4 hours)(Lane 4), Hep G2 (Lane 5), Hep G2 (Protein Transport Inhibitor Cocktail (500X), 1X, 4 hours)(Lane 6) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded human liver tissue. To expose target proteins, 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a CTGF monoclonal antibody (Product # MA5-26818).
