Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- MAB91901-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat CTGF/CCN2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human CTGF/CCN2 in direct ELISAs. Detects human, mouse, and rat CTGF/CCN2 in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
P29279
- Isotype
- IgG
- Antibody clone number
- 2191A
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human, Mouse, and Rat CTGF/CCN2 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells, SVEC4-10 mouse vascular endothelial cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human/Mouse/Rat CTGF/CCN2 Monoclonal Antibody (Catalog # MAB91901) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for CTGF/CCN2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.