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- Product number
- 459160 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cytochrome C Antibody Cocktail
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- ApoTrack™ Cytochrome c WB Cocktail (Sku# 459160) provides a means to detect apoptosis while detecting release of cytochrome c, as well as a cytoplasmic and mitochondrial markers with the help of western blot. This cocktail provides a means to detect cytochrome c, as well as a cytoplasmic and mitochondrial markers
- Antibody clone number
- Cocktail
- Concentration
- 0.9 mg/mL
Submitted references The Coxiella burnetii type IV secretion system substrate CaeB inhibits intrinsic apoptosis at the mitochondrial level.
Klingenbeck L, Eckart RA, Berens C, Lührmann A
Cellular microbiology 2013 Apr;15(4):675-87
Cellular microbiology 2013 Apr;15(4):675-87
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of Cytochrome C in Jurkat, 143B, and HeLa cells using a Cytocrome C Monoclonal antibody (Product # 459160). Apoptosis was induced in Jurkat, 143B osteosarcoma, and HeLa cells bia treatment with FAS and staurosporine. Isolation of Mitochondrial and cytoplasmic fractions were performed before detecting with the Cyctocrome C Monoclonal antibody (Product # 459160). The western blot shows that Cytochrome c has translocated partially in FAS-induced cells and STS-treated 43B osteosarcoma cells, and almost completely in STS-treated Jurkat and HeLa cells.
