Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- BAF2265 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal FGFR4 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse FGF R4 in Western blots. In Western blots, approximately 35% cross-reactivity with recombinant human (rh) FGF R4 is observed and less than 5% cross-reactivity with rhFGF R1 alpha (IIIb), rhFGF R1 alpha (IIIc), rhFGF R1 beta (IIIb), rhFGF R1 beta (IIIc), recombinant mouse (rm) FGF R2 alpha (IIIb), rmFGF R2 alpha (IIIc), rmFGF R2 beta (IIIb), rmFGF R2 beta (IIIc), rmFGF R5, and rmFGF R5 beta is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Biotin
- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- FGF R4 in Mouse Embryo. FGF R4 was detected in immersion fixed frozen sections of mouse embryo (E11.5) using Goat Anti-Mouse FGF R4 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2265) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the epidermis. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.