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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- orb11605 - Provider product page
- Provider
- Biorbyt
- Product name
- GAPDH antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal to GAPDH (glyceraldehyde 3-phosphate dehydrogenase). GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains.
- Reactivity
- Human, Mouse, Rat, Canine, Simian
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 μg
- Concentration
- 1 mg per ml
- Storage
- Store at -20 °C. for long term storage and 2-8 °C. for up to one month.Avoid freeze/thaw cycles.
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Supportive validation
- Submitted by
- Biorbyt (provider)
- Main image
- Experimental details
- Western blot analysis of 293 HEK cell lysate using GAPDH antibody
- Other comments
- Specificity: Detects a band of 37 kDa by Western blot in the following human (293A, HMEC-1, U-118, HaCat), rat (TR-iBRB), mouse (AtT-20, Hepa), Dog (D17) and monkey (COS-7) whole cell lysates.
Supportive validation
- Submitted by
- Biorbyt (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Hepa1-6 cells using GAPDH antibody at 1:50 dilution.
- Other comments
- Specificity: Detects a band of 37 kDa by Western blot in the following human (293A, HMEC-1, U-118, HaCat), rat (TR-iBRB), mouse (AtT-20, Hepa), Dog (D17) and monkey (COS-7) whole cell lysates.
