AMAb91152

antibody from Atlas Antibodies

Targeting: GAPDH GAPD

Western blot (Enhanced validation)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AMAb91152

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#AMAb91152, RRID:AB_2665821

Product name

Anti-GAPDH

Antibody type

Monoclonal

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

TVKAENGKLVINGNPITIFQERDPSKIKWGDAGAE
YVVESTGVFTTMEKAGAHLQGGAKRVIIS


Epitope

Binds to an epitope located within the peptide sequence ERDPSKIKWGDAGAE as determined by overlapping synthetic peptides.

Isotype

IgG

Antibody clone number

CL3265

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-GAPDH antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431, human cell line HepG2, mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex shows immunoreactivity in neuropil as well as strong cytoplasmic positivity in a subset of cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney shows strong cytoplasmic and nuclear positivity in renal tubules and glomeruli.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human rectum shows strong cytoplasmic and nuclear positivity in glandular and connective tissue cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human liver shows strong cytoplasmic and nuclear immunoreactivity in hepatocytes.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle shows moderate cytoplasmic and strong nuclear immunoreactivity in muscle fibers.