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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AM11013SU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM11013SU-N, RRID:AB_1752704
- Product name
- anti GAPDH
- Antibody type
- Monoclonal
- Antigen
- GAPDH recombinant protein is used to produce this monoclonal antibody.
- Reactivity
- Human
- Host
- Mouse
- Antibody clone number
- 1A10A10
- Vial size
- 0.1 ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of anti-GAPDH Antibody Cat.-No AM11013SU-NÂ in HepG2, A375, A2058, NCI-H460 and CEM cell line lysates (35ug/lane). GAPDH(arrow) was detected using the Mab ascites (1/25000 dilution).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of GAPDH Antibody Cat.-No AM11013SU-N with hela cell followed by Alexa Fluor®488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with GAPDH Antibody Cat.-No AM11013SU-N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
