53-9768-80

antibody from Invitrogen Antibodies

Targeting: HSPA5 BiP, GRP78

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

53-9768-80

Provider

Invitrogen Antibodies

Product name

GRP78 Monoclonal Antibody (C38), Alexa Fluor™ 488, eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: The monoclonal antibody C38 recognizes human glucose-related protein 78 (GRP78). The GRP78 is encoded by the HSPA5 gene and functions as an endoplasmic reticulum (ER) chaperone protein and is a member of the heat shock protein 70 family. GRP78 functions within the ER lumen to facilitate proper protein folding, to prevent intermediates from aggregating, to target misfolded proteins for degradation, and as a calcium binding protein capable of recognizing ER stress and inducing the Unfolded Protein Response (UPR). During normal cellular function, GRP78 is bound to the transmembrane sensor proteins PERK, IRE1, and ATF6, keeping them in an inactive state. During ER stress, GRP78 binds to the hydrophobic residues of unfolded proteins within the ER and releases the sensor proteins, allowing signaling to the nucleus and the initiation of the UPR. During the UPR, protein synthesis is slowed and the transcription of ER chaperones is upregulated, increasing the capacity of the ER.

Conjugate

Green dye

Antibody clone number

C38

Concentration

0.5 mg/mL

References

Submitted references

Synthetic Antibody Mimics Based on Cancer-Targeting Immunostimulatory Peptides.

Descalzi-Montoya D, Montel RA, Smith K, Dziopa E, Darwich A, Yang Z, Bitsaktsis C, Korngold R, Sabatino D
Chembiochem : a European journal of chemical biology 2021 May 4;22(9):1589-1596

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Cell surface GRP78 regulates BACE2 via lysosome-dependent manner to maintain mesenchymal phenotype of glioma stem cells.

Chen Z, Wang H, Zhang Z, Xu J, Qi Y, Xue H, Gao Z, Zhao R, Wang S, Zhang S, Qiu W, Guo X, Li G
Journal of experimental & clinical cancer research : CR 2021 Jan 7;40(1):20

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry of methanol-fixed MCF7 cells using 30 µg/mL of Mouse IgG1 K Isotype Control Alexa Fluor® 488 (left) or 30 µg/mL of Anti-Human GRP78 Alexa Fluor® 488 (right). Nuclei are stained with DAPI.

Conjugate

Green dye

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 6 Blocking csGRP78 induces lysosomal degradation of BACE2. a Co-immunofluorescence staining of csGRP78 (red) and BACE2 (green) in GSC20 and 267. Scale bar, 25 mum. b Colocalization analysis for co-expression of BACE2 and csGRP78 in cell membrane of GSC20 and 267 using colocalization finder plugin. c Western blotting for BACE2 in GSC267 with anti-GRP78 treatment for 72 h or lentiviral shGRP78 expressing. d BACE2 RNA level in GSC20 and 267 was detected by qRT-PCR with anti-GRP78 treatment or lentiviral shGRP78 expressing, GADPH as the reference gene. e Western blotting for BACE2 protein in GSC267 that treated with MG-132, Chloroquine (CQ) or anti-GRP78, respectively, and co-treatment of anti-GRP78 with MG-132 or CQ. f and g Upper, the confocal microscopy images for overview and splitting channel of BACE2 (red) and LAMP1 (green). The yellow triangles indicate the overlapped regions (yellow). Lower, the analysis plot for the fluorescence intensity of two channels. Red line for BACE2 and green line for LAMP1. Scale bar, 5 mum, and 25 mum for the large field of view. Error bar indicates at least three independent experiments and data are shown as mean +- SD

Conjugate

Green dye