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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- MA5-15619 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GRP78 Monoclonal Antibody (4E3)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15619 targets HSPA5 in IHC and WB applications and shows reactivity with Human samples.
- Antibody clone number
- 4E3
- Concentration
- Conc. Not Determined
Submitted references A Pipeline for Natural Small Molecule Inhibitors of Endoplasmic Reticulum Stress.
Correia da Silva D, Valentão P, Andrade PB, Pereira DM
Frontiers in pharmacology 2022;13:956154
Frontiers in pharmacology 2022;13:956154
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of MCF7 (Lane 1), MCF7 treated with Thapsigargin (1uM for 24hr) (Lane 2), HeLa (Lane 3), HeLa treated with Thapsigargin (1uM for 24hr) (Lane 4), MOLT-4 (Lane 5), MOLT-4 treated with Thapsigargin (1uM for 24hr) (Lane 6). The blot was probed with Anti-GRP78 Monoclonal Antibody (Product # MA5-15619, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). A 78 kDa band corresponding to GRP78 was observed across cell lines tested and was enhanced upon Thapsigargin treatment in all the cell lines.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GRP78/HSPA5 using GRP78/HSPA5 monoclonal antibody (Product # MA5-15619) in NIH/3T3 (1), HeLa (2) and Jurkat (3) cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human thyroid tissues using GRP78/HSPA5 monoclonal antibody (Product # MA5-15619).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 5 (A) Impact of delphinidin, fisetin, myristic acid, sennoside B and quercetin-3- O -beta-D-glucoside on Abeta 25-35 -induced protein aggregation in MRC-5 cells, determined by the fluorescence of thioflavin T. Results correspond to the mean +- standard error of the mean of, at least, three independent experiments, individually performed in triplicate. (B) The protective effect of bioactive molecules was captured under a fluorescence microscope. The fluorescence signal of thioflavin T is represented in the histograms. (C) Effect of delphinidin on Tg-induced BiP expression in MRC-5 cells, as evaluated by Western blotting. Results correspond to the mean +- standard error of the mean of, at least, three independent experiments. (D) Images obtained through Western blotting analysis.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 6 (A) Effect of delphinidin and fisetin on Abeta 25-35 -induced protein aggregation in SH-SY5Y cells, as evaluated by determination of the fluorescence of thioflavin T. Results correspond to the mean +- standard error of the mean of, at least, three independent experiments, each performed in triplicate. (B) The effect of bioactive molecules was observed under a fluorescence microscope. The fluorescence signal of thioflavin T is represented in the histograms. (C) Effect of delphinidin on Tg-induced BiP expression in SH-SY5Y cells, as evaluated by Western blotting. Results correspond to the mean +- standard error of the mean of, at least, three independent experiments. (D) Example of the obtained Western blotting results.
