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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PA5-32437 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GRP78 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, chicken, canine, amphibian, mouse and rat based on sequence homology.
Submitted references Sustained HSP25 Expression Induces Clasmatodendrosis via ER Stress in the Rat Hippocampus.
Endothelial NOS activation induces the blood-brain barrier disruption via ER stress following status epilepticus.
Endoplasmic reticulum (ER) stress protein responses in relation to spatio-temporal dynamics of astroglial responses to status epilepticus in rats.
Kim JE, Hyun HW, Min SJ, Kang TC
Frontiers in cellular neuroscience 2017;11:47
Frontiers in cellular neuroscience 2017;11:47
Endothelial NOS activation induces the blood-brain barrier disruption via ER stress following status epilepticus.
Ko AR, Kim JY, Hyun HW, Kim JE
Brain research 2015 Oct 5;1622:163-73
Brain research 2015 Oct 5;1622:163-73
Endoplasmic reticulum (ER) stress protein responses in relation to spatio-temporal dynamics of astroglial responses to status epilepticus in rats.
Ko AR, Kim JY, Hyun HW, Kim JE
Neuroscience 2015 Oct 29;307:199-214
Neuroscience 2015 Oct 29;307:199-214
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-GRP78 Polyclonal Antibody (Product # PA5-32437) and a 72 kDa band corresponding to GRP78 was observed across cell lines and tissue extracts tested and increased upon Thapsigargin treatment. Whole cell extracts (30 µg lysate) of MCF-7 (Lane 1), MCF-7 treated with Thapsigargin (1uM for 24 Hours) (Lane 2), HeLa (Lane 3) and HeLa treated with Thapsigargin (1uM for 24 Hours) (Lane 4), MOLT-4 (Lane 5), Hep G2 (Lane 6), tissue extracts of Mouse Liver (Lane 7) and Mouse Pancreas (Lane 8) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of GRP78 using anti-GRP78 Polyclonal Antibody (Product # PA5-32437) in Breast Carcinoma Cancer Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:100.
