Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [4]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- GTX79247 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX79247, RRID:AB_11166571
- Product name
- HSP90 beta antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- WB analysis of indicated cell lysates (50ug per lane) HSP84 antibody at a dilution of 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ICC/IF analysis of HepG2 cells with (left) or without (right) using HSP84 antibody at a dilution of 1:100 (green). F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
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- GeneTex (provider)
- Main image
- Experimental details
- ICC/IF analysis of U251 cells with (green) or without (left) HSP84 antibody at a dilution of 1:100 (green). F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
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- GeneTex (provider)
- Main image
- Experimental details
- ICC/IF analysis of HeLa and NIH3T3 cells using HSP84 antibody at a dilution of 1:50 (green) and Hoechst 33342 (blue). Images were taken at 20X magnification.
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- GeneTex (provider)
- Main image
- Experimental details
- ICC/IF analysis of A2058 cells with (left) or without (right) HSP84 antibody at a dilution of 1:100 (green). F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- IP analysis was performed on 500 ug of HeLa extract using 2 ug of HSP84 antibody. The precipitated protein was detected by the same antibody at a dilution of 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IHC-P analysis of human breast carcinoma with (left) or without (right) HSP84 antibody at a dilution of 1:100. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IHC-P analysis of human placenta tissue with (left) or without (right) HSP84 antibody at a dilution of 1:20. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IHC-P analysis of human tonsil tissue with (left) or without (right) HSP84 antibody at a dilution of 1:100. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes.