PA5-21756

antibody from Invitrogen Antibodies

Targeting: SET 2PP2A, IGAAD, IPP2A2, PHAPII, TAF-I, TAF-IBETA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21756

Provider

Invitrogen Antibodies

Product name

SET Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Recommended positive controls: 293T, A431, HeLa, HepG2.

Concentration

0.959 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SET using A) 30 µg 293T whole cell lysate and B) 30 µg whole cell lysate of human SET-transfected 293T cells. Samples were loaded onto a 12% SDS-PAGE gel and probed with a SET polyclonal antibody (Product # PA5-21756) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of SET was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a SET Polyclonal Antibody (Product # PA5-21756) at a dilution of 1:1000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of SET was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: SET Polyclonal Antibody (Product # PA5-21756) diluted at 1:200. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using SET antibody, isoform 2 specific (Product # PA5-21756) at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.